Professor Andrew Peden
School of Biosciences
Professor
+44 114 222 2312
Full contact details
School of Biosciences
D06, Florey Building
Florey Building
Western Bank
Sheffield
S10 2TN
- Profile
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- 2023-present: Professor, School of Bioscience, the º£½ÇÉçÇø of Sheffield.
- 2012-2018: Lecturer, Department of Biomedical Science, the º£½ÇÉçÇø of Sheffield.
- 2019-present: Senior Lecturer, School of Biosciences, the º£½ÇÉçÇø of Sheffield.
- 2012-2018: Lecturer, Department of Biomedical Science, the º£½ÇÉçÇø of Sheffield
- 2006-2012: Principal Investigator, º£½ÇÉçÇø of Cambridge (MRC CDA).
- 2001-2005: Postdoctoral fellow, Genentech Inc.
- 2000-2001: Postdoctoral fellow, Stanford º£½ÇÉçÇø (Wellcome Trust Research Fellowship).
- 1995-1999: PhD, º£½ÇÉçÇø of Cambridge.
- 1991-1995: BSc., º£½ÇÉçÇø of Edinburgh.
- Research interests
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Constitutive secretion is a conserved process required for the delivery of newly synthesised proteins and lipids to plasma membrane as well as the exocytocis of extracellular factors such as cytokines, lipoproteins and antibodies.
My lab is interested in identifying and characterising the pathways and machinery involved in constitutive secretion.
Elucidating the post-Golgi pathways and machinery required for constitutive secretion
Constitutive secretion is required for many biologically important processes, such as inflammation (cytokine secretion), adaptive immunity (IgG secretion), tissue remodelling (extracellular matrix secretion) and cholesterol homeostasis (lipoprotein particle secretion). Perturbations in the secretion of these factors can cause disease.
For example dysregulation of antibody secretion plays a significant role in the development of diseases such Amyloidosis, Macroglobulinemia and Monoclonal Gammopathies. Thus having the ability to modulate the secretion of various cellular factors has significant therapeutic potential.
My lab has 3 main aims:
1) To elucidate the role of SNAREs in constitutive secretion
SNAREs are a familyof proteins required for the fusion of membranes and there are 38 encoded in the human genome. Each SNARE is localised to a specific compartment within the cell and required a defined set of fusion steps.
However, it is not known which SNAREs are required for the fusion of secretory vesicles with the plasma membrane. To address this we have developed novel assays for measuring constitutive secretion and have used them in conjunction with targeted siRNA screens in both mammalian and Drosophila based systems.
We have shown that in mammals the post-Golgi SNAREs SNAP29 and STX19 and are required for constitutive secretion. SNAP29 is mutated in the human disorder CEDNIK so our work may help shed light on the molecular details of this disease.
Many questions remain regarding the role of SNAREs in constitutive secretion. For example, how are the functions of SNAP29 and STX19 coordinated, how is constitutive secretion regulated, how are SNAREs packaged into post-Golgi secretory carriers. We are addressing these questions using live cell imaging, secretion assays, proteomics and structural based approaches.
Movie showing RPE-1 cells secreting the GFP tagged protein we use to measure secretion. The tubular structures emanating from the centre of the cells are secretory carriers which will eventually fuse with the plasma.
2) To identify and characterise novel machinery required for post-Golgi trafficking and antibody secretion
Very little is known about the machinery required for budding, transporting, docking and fusing post-Golgi transport vesicles. To identify this machinery we are using two approaches.
Firstly we are using a screening approach (RNAi or chemical) where we will make use of our novel secretion assays in mammalian and Drosophila cell lines. In the second approach we plan to use proteomics to quantify changes in protein expression associated with plasma cell differentiation.
3) To understand the molecular interactions required for post-Golgi trafficking and secretion
To elucidate the molecular interactions involved in post-Golgi trafficking and secretion we are tacking a structural approach in collaboration with Professor David Owen.
We are particularly interested in understanding how post-Golgi SNAREs are packaged into transport vesicles.
We have recently shown that the clathrin coated vesicle protein CALM directly binds to the coiled-coil domains of the R-SNAREs VAMPs 2/3/8 and facilitates their internalisation.
- Publications
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Featured publications
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Journal articles
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Conference proceedings papers
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- Research group
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Collaborators
- David James (º£½ÇÉçÇø of Sheffield)
- Mark Collins (º£½ÇÉçÇø of Sheffield)
- Bazbek Davletov (º£½ÇÉçÇø of Sheffield)
- Luke Chamberlain (º£½ÇÉçÇø of Strathclyde)
- Clare Futter (º£½ÇÉçÇø College London)
PhD studentship opportunities
We advertise PhD opportunities (Funded or Self-Funded) on FindAPhD.com
- Grants
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- BBSRC
- MRC
- Medimmune
- UCB
- Teaching activities
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- BIS213: Skills in Biomedical Science (Herceptin practical co-ordinator).
- BIS225: Molecular and Cell Biology.
- BMS376: Membrane Dynamics in Health and Disease.
- Supervise level 3 research projects.
- Supervise level 4 and masters research projects.
- Professional activities and memberships
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- 2023-present BBSRC Committee D Core Member
- 2021-present Member of the WHO COVID-19 assays group
- 2018-present Carnegie Trust research assessor
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